diff --git a/tests/e2e/conftest.py b/tests/e2e/conftest.py
index 15c6d02e1b9..08df334d4b8 100644
--- a/tests/e2e/conftest.py
+++ b/tests/e2e/conftest.py
@@ -20,7 +20,6 @@ from typing import Iterator
 
 import pytest
 import requests
-from dotenv import load_dotenv
 
 from e2e_config import CONTROL_PLANE_BASE_URL, PROXY_BASE_URL
 from lifecycle import GatewayProvider, ResourceManager
@@ -30,7 +29,6 @@ _E2E_TEST_RAN = pytest.StashKey[bool]()
 
 
 def pytest_configure(config: pytest.Config) -> None:
-    load_dotenv(Path(__file__).parent / ".env")
     config.addinivalue_line(
         "markers",
         "e2e: live test that requires a running proxy and real provider keys",