diff --git a/litellm/llms/bedrock/files/transformation.py b/litellm/llms/bedrock/files/transformation.py
index 700f448a913..3007b54808c 100644
--- a/litellm/llms/bedrock/files/transformation.py
+++ b/litellm/llms/bedrock/files/transformation.py
@@ -403,12 +403,13 @@ class BedrockFilesConfig(BaseAWSLLM, BaseFilesConfig):
             data=create_file_data,
         )
 
-        # If s3_region_name is set and aws_region_name is not, propagate it so
-        # SigV4 signing uses the correct region (e.g. GovCloud us-gov-west-1).
+        # s3_region_name always wins for S3 operations (same priority as in
+        # get_complete_file_url above). Overwrite aws_region_name unconditionally
+        # so the SigV4 region matches the URL region, avoiding SignatureDoesNotMatch.
         s3_region_name = litellm_params.get("s3_region_name") or optional_params.get(
             "s3_region_name"
         )
-        if s3_region_name and not optional_params.get("aws_region_name"):
+        if s3_region_name:
             optional_params = {**optional_params, "aws_region_name": s3_region_name}
 
         # Sign the request and return a pre-signed request object
diff --git a/tests/test_litellm/llms/bedrock/files/test_bedrock_files_transformation.py b/tests/test_litellm/llms/bedrock/files/test_bedrock_files_transformation.py
index aec1d164fe2..40a17c12118 100644
--- a/tests/test_litellm/llms/bedrock/files/test_bedrock_files_transformation.py
+++ b/tests/test_litellm/llms/bedrock/files/test_bedrock_files_transformation.py
@@ -278,8 +278,6 @@ class TestBedrockFilesTransformation:
         Previously the code fell back to us-west-2 even when s3_region_name was set,
         breaking GovCloud (us-gov-west-1) deployments.
         """
-        from unittest.mock import MagicMock, patch
-
         from litellm.llms.bedrock.files.transformation import BedrockFilesConfig
 
         config = BedrockFilesConfig()
@@ -329,7 +327,7 @@ class TestBedrockFilesTransformation:
         When s3_region_name is provided, transform_create_file_request must pass
         that region to _sign_s3_request so SigV4 signatures use the correct region.
         """
-        from unittest.mock import MagicMock, patch
+        from unittest.mock import patch
 
         from litellm.llms.bedrock.files.transformation import BedrockFilesConfig
 
@@ -376,6 +374,62 @@ class TestBedrockFilesTransformation:
             "s3_region_name must be forwarded as aws_region_name for SigV4 signing"
         )
 
+    def test_s3_region_name_wins_over_aws_region_name_for_signing(self):
+        """
+        When both s3_region_name and aws_region_name are set to different values,
+        s3_region_name must win for signing (same as for the URL). Otherwise the
+        SigV4 signature would be computed against a different region than the URL,
+        causing SignatureDoesNotMatch from AWS.
+        """
+        from unittest.mock import patch
+
+        from litellm.llms.bedrock.files.transformation import BedrockFilesConfig
+
+        config = BedrockFilesConfig()
+
+        jsonl_content = json.dumps(
+            {
+                "custom_id": "req-1",
+                "method": "POST",
+                "url": "/v1/chat/completions",
+                "body": {
+                    "model": "bedrock/amazon.nova-pro-v1:0",
+                    "messages": [{"role": "user", "content": "Hello"}],
+                    "max_tokens": 10,
+                },
+            }
+        ).encode()
+
+        create_file_data = {
+            "file": ("batch.jsonl", jsonl_content, "application/jsonl"),
+            "purpose": "batch",
+        }
+
+        litellm_params = {
+            "s3_bucket_name": "litellm-batch-352026",
+            "s3_region_name": "us-gov-west-1",
+        }
+        # aws_region_name set to something different — s3_region_name must still win
+        optional_params = {"aws_region_name": "us-east-1"}
+
+        captured_optional_params: dict = {}
+
+        def fake_sign(content, api_base, optional_params):
+            captured_optional_params.update(optional_params)
+            return {"Authorization": "fake"}, content
+
+        with patch.object(config, "_sign_s3_request", side_effect=fake_sign):
+            config.transform_create_file_request(
+                model="amazon.nova-pro-v1:0",
+                create_file_data=create_file_data,
+                optional_params=optional_params,
+                litellm_params=litellm_params,
+            )
+
+        assert captured_optional_params.get("aws_region_name") == "us-gov-west-1", (
+            "s3_region_name must override aws_region_name for SigV4 signing"
+        )
+
     def test_openai_passthrough_still_works(self):
         """
         Regression test: ensure OpenAI-compatible models (e.g. gpt-oss)