diff --git a/tests/vector_store_tests/rag/test_rag_s3_vectors.py b/tests/vector_store_tests/rag/test_rag_s3_vectors.py
new file mode 100644
index 00000000000..17fcf895063
--- /dev/null
+++ b/tests/vector_store_tests/rag/test_rag_s3_vectors.py
@@ -0,0 +1,107 @@
+"""
+S3 Vectors RAG ingestion tests.
+
+Requires environment variables:
+- AWS_ACCESS_KEY_ID
+- AWS_SECRET_ACCESS_KEY
+- AWS_REGION_NAME (optional, defaults to us-west-2)
+
+Optional:
+- S3_VECTOR_BUCKET_NAME (optional, auto-generates if not set)
+"""
+
+import os
+import sys
+from typing import Any, Dict, Optional
+
+import pytest
+
+sys.path.insert(0, os.path.abspath("../../.."))
+
+import litellm
+from litellm.types.rag import RAGIngestOptions
+from tests.vector_store_tests.rag.base_rag_tests import BaseRAGTest
+
+
+class TestRAGS3Vectors(BaseRAGTest):
+    """Test RAG Ingest with AWS S3 Vectors."""
+
+    @pytest.fixture(autouse=True)
+    def check_env_vars(self):
+        """Check required environment variables before each test."""
+        aws_key = os.environ.get("AWS_ACCESS_KEY_ID")
+        aws_secret = os.environ.get("AWS_SECRET_ACCESS_KEY")
+
+        if not aws_key or not aws_secret:
+            pytest.skip("Skipping S3 Vectors test: AWS credentials required")
+
+    def get_base_ingest_options(self) -> RAGIngestOptions:
+        """
+        Return S3 Vectors-specific ingest options.
+
+        Chunking is configured via chunking_strategy (unified interface).
+        Embeddings are generated using LiteLLM's embedding API.
+        """
+        vector_bucket_name = os.environ.get(
+            "S3_VECTOR_BUCKET_NAME", "test-litellm-vectors"
+        )
+        aws_region = os.environ.get("AWS_REGION_NAME", "us-west-2")
+
+        return {
+            "chunking_strategy": {
+                "chunk_size": 512,
+                "chunk_overlap": 100,
+            },
+            "embedding": {
+                "model": "text-embedding-3-small"  # Can use any LiteLLM-supported model
+            },
+            "vector_store": {
+                "custom_llm_provider": "s3_vectors",
+                "vector_bucket_name": vector_bucket_name,
+                "index_name": "test-index",
+                "dimension": 1536,  # text-embedding-3-small dimension
+                "distance_metric": "cosine",
+                "non_filterable_metadata_keys": ["source_text"],
+                "aws_region_name": aws_region,
+            },
+        }
+
+    async def query_vector_store(
+        self,
+        vector_store_id: str,
+        query: str,
+    ) -> Optional[Dict[str, Any]]:
+        """Query S3 Vectors index."""
+        try:
+            # Import the ingestion class to use its query method
+            from litellm.rag.ingestion.s3_vectors_ingestion import (
+                S3VectorsRAGIngestion,
+            )
+        except ImportError:
+            pytest.skip("S3 Vectors ingestion not available")
+
+        vector_bucket_name = os.environ.get(
+            "S3_VECTOR_BUCKET_NAME", "test-litellm-vectors"
+        )
+        aws_region = os.environ.get("AWS_REGION_NAME", "us-west-2")
+
+        # Create ingestion instance to use query method
+        ingest_options = {
+            "embedding": {"model": "text-embedding-3-small"},
+            "vector_store": {
+                "custom_llm_provider": "s3_vectors",
+                "vector_bucket_name": vector_bucket_name,
+                "aws_region_name": aws_region,
+            },
+        }
+
+        ingestion = S3VectorsRAGIngestion(ingest_options=ingest_options)
+
+        # Query the index
+        results = await ingestion.query_vector_store(
+            vector_store_id=vector_store_id,
+            query=query,
+            top_k=5,
+        )
+
+        return results