diff --git a/litellm/integrations/langfuse/langfuse_otel.py b/litellm/integrations/langfuse/langfuse_otel.py
index 0117348da66..4be89e4011d 100644
--- a/litellm/integrations/langfuse/langfuse_otel.py
+++ b/litellm/integrations/langfuse/langfuse_otel.py
@@ -94,6 +94,21 @@ def _caller_tag_list(value: object) -> tuple[str, ...]:
     return ()
 
 
+def _end_user_without_request_user(kwargs: Mapping[str, object]) -> str | None:
+    """The proxy end-user id (header mapping, ``x-litellm-end-user-id``, ...) when the request carries no ``user``.
+
+    The vanilla Langfuse logger uses this id as the trace user; the shared OTel attributes only fall back to the
+    key owner's ``user_api_key_user_id``, so without this every such call lands on one Langfuse user."""
+    payload: Final = as_str_mapping(kwargs.get("standard_logging_object")) or {}
+    metadata: Final = as_str_mapping(payload.get("metadata")) or {}
+    end_user: Final = metadata.get("user_api_key_end_user_id")
+    if not end_user:
+        return None
+    optional_params: Final = as_str_mapping(kwargs.get("optional_params")) or {}
+    model_params: Final = as_str_mapping(payload.get("model_parameters")) or {}
+    return None if optional_params.get("user") or model_params.get("user") else str(end_user)
+
+
 def _parent_of(span: object) -> trace.SpanContext | None:
     parent: Final = getattr(span, "parent", None)
     return parent if isinstance(parent, trace.SpanContext) else None
@@ -158,7 +173,7 @@ class LangfuseOtelLogger(OpenTelemetry):
         super().__init__(config=config, *args, **kwargs)
 
     @staticmethod
-    def set_langfuse_otel_attributes(span: Span, kwargs, response_obj):
+    def set_langfuse_otel_attributes(span: Span, kwargs: Mapping[str, object], response_obj: object) -> None:
         """
         Sets OpenTelemetry span attributes for Langfuse observability.
         Uses the same attribute setting logic as Arize Phoenix for consistency.
@@ -306,6 +321,11 @@ class LangfuseOtelLogger(OpenTelemetry):
                 langfuse_environment,
             )
 
+        # Before the metadata mapping, so an explicit `trace_user_id` still wins.
+        end_user: Final = _end_user_without_request_user(kwargs)
+        if end_user is not None:
+            safe_set_attribute(span, LangfuseSpanAttributes.TRACE_USER_ID.value, end_user)
+
         metadata: Final = LangfuseOtelLogger._extract_langfuse_metadata(kwargs)
         LangfuseOtelLogger._set_metadata_attributes(span=span, metadata=metadata)
 
diff --git a/tests/unit/integrations/test_langfuse_otel.py b/tests/unit/integrations/test_langfuse_otel.py
index 71087b4de51..3c10414f4bb 100644
--- a/tests/unit/integrations/test_langfuse_otel.py
+++ b/tests/unit/integrations/test_langfuse_otel.py
@@ -311,6 +311,51 @@ class TestLangfuseOtelIntegration:
                 actual == expected
             ), "Mismatch between expected and actual OTEL attribute mapping."
 
+    @pytest.mark.parametrize(
+        "end_user, optional_params, metadata, expected_user",
+        [
+            ("agent-a", {}, {}, "agent-a"),
+            ("agent-a", {"user": "body-user"}, {}, "body-user"),
+            ("agent-a", {}, {"trace_user_id": "explicit-user"}, "explicit-user"),
+            (None, {}, {}, "default_user_id"),
+        ],
+        ids=[
+            "end-user-replaces-key-owner-fallback",
+            "request-user-still-wins",
+            "explicit-trace-user-id-still-wins",
+            "without-end-user-key-owner-fallback-unchanged",
+        ],
+    )
+    def test_trace_user_falls_back_to_end_user(
+        self,
+        end_user: str | None,
+        optional_params: dict[str, str],
+        metadata: dict[str, str],
+        expected_user: str,
+    ) -> None:
+        """Like the vanilla Langfuse logger, the proxy end user (e.g. from `user_header_mappings`) is the trace user
+        when the request carries no `user`, instead of the key owner's `user_api_key_user_id`."""
+        from litellm.types.integrations.langfuse_otel import LangfuseSpanAttributes
+
+        exporter: Final = InMemorySpanExporter()
+        provider: Final = TracerProvider()
+        provider.add_span_processor(SimpleSpanProcessor(exporter))
+        span: Final = provider.get_tracer(__name__).start_span("litellm_request")
+        logging_metadata: Final[dict[str, str]] = {"user_api_key_user_id": "default_user_id"}
+        if end_user is not None:
+            logging_metadata["user_api_key_end_user_id"] = end_user
+        kwargs: Final[dict[str, object]] = {
+            "optional_params": optional_params,
+            "litellm_params": {"metadata": metadata},
+            "standard_logging_object": {"metadata": logging_metadata, "model_parameters": optional_params},
+        }
+
+        LangfuseOtelLogger.set_langfuse_otel_attributes(span, kwargs, None)
+        span.end()
+
+        attributes: Final = exporter.get_finished_spans()[0].attributes or {}
+        assert attributes.get(LangfuseSpanAttributes.TRACE_USER_ID.value) == expected_user
+
     @pytest.mark.parametrize(
         "metadata, expected_version",
         [